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ATCC CCL-185) cell lines were cultured at 37 C, 5% CO 2 in DMEM with 4.5 g/l of glucose, 1% penicillin/streptomycin (P/S) and 10% of heat-inactivated fetal bovine serum (FBS) (Gibco BRL, Gaithersburg, Md.)

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It should be noted that the applicability of photo-controlled LYTACs is inherently limited by the restricted tissue penetration of light, which confines their use primarily to superficial tumors or those accessible through endoscopic or intraoperative illumination